Production of Ultrapure Water for RNase-Sensitive Applications

In this application note removal of RNases by ultrafiltration is compared to chemical inactivation by DEPC treatment. Ultrafiltration cartridges included in EMD Millipore water purification systems are an effective alternative to DEPC treatment allowing delivery of highpurity RNase-free water on demand (based on the results of several experiments on RNA preparation, RNA digestion, an RNA stability test and dosage of RNases).