Profiling Analysis of Mono-, Di- and Tri-phosphate Nucleotides Using Capillary Ion Chromatography Mass Spectrometry

There have been extensive studies of using nucleoside analogs as prodrugs in anti-cancer, anti-viral and immunosuppressive therapy, and monitoring of their activated nucleotides metabolites is of paramount importance to understand the pharmacology. This poster evaluates a quantitative method for targeted metabolic profiling analysis of mono-, di-, and tri-phosphate nucleotides using capillary ion chromatography tandem mass spectrometry (Cap IC-MS-MS).