Proof of Concept for the Quantitation of Peptides in Human Plasma by Liquid Chromatography-Multiple Reaction Monitoring (LC-MRM)

This work describes “proof of concept” data supporting sensitive and reproducible quantitative measurements of peptides in human plasma by multiple reaction monitoring (MRM) coupled with stable isotope dilution (SID)-MS. To demonstrate the specificity, sensitivity, and reproducibility of an LC-MRM assay, peptides and internal standards were spiked into human serum albumin and IgG depleted plasma.