Protein A Affinity IgG Capture Followed by AdvanceBio SEC Aggregation Analysis

This application note describes the quantification of aggregates in biotherapeutic samples using the Agilent InfinityLab 2D-LC solutions. Protein A affinity capture was performed in the first dimension, and size exclusion chromatography in the second dimension. Multiple heart-cutting was used to transfer the samples from the first to the second dimension. This technique enabled the entire characterization to be done with a single analysis, eliminating the need for fraction collection and multiple injections.