Protein barcodes for Next-Generation Protein Sequencing™

Explore the transformative capabilities of barcoding technologies in biotechnology, especially when integrated with next-generation DNA sequencing. These technologies facilitate diverse applications such as tracking sample identity, achieving single-cell or spatial resolution in transcriptomic studies, and enriching genotypes in protein engineering. In this application note, discover Quantum-Si's Next-Generation Protein Sequencing™ (NGPS) on the Platinum^® instrument, a novel protein barcoding approach presented in the note demonstrates the design and sequencing of distinguishable synthetic peptides, statistical analysis for barcode design criteria, and a method for enzymatic library preparation of recombinantly expressed peptide barcodes.