Protein Extraction from Cypress Pollen with Minilys

The common cypress pollen (Cupressus sempervirens) is becoming an increasing cause of respiratory allergy in Europe but because of its particular structural features and physico-chemical composition, cypress pollen is one of the most pollen difficult to analyze in terms of protein content and therefore allergens. Dry milling may be a good alternative for the extraction of cypress pollen proteins and to generate fragments of smaller sizes for experiments and ultrastructural analysis of immuno-reactivity.