Protein sizing and quantification using the Agilent ProteoAnalyzer System

In this application note from Agilent Technologies, explore the reproducibility of protein sizing and quantification with the ProteoAnalyzer, an automated quality control instrument which analyzes proteins based on migration time and fluorescence intensity using parallel capillary electrophoresis with sodium dodecyl-sulfate (CE-SDS). The system has the ability to analyze up to 12 samples in one run in approximately 30 minutes. Sizing accuracy and precision is demonstrated using bovine serum albumin and carbonic anhydrase II. The samples will also be used to provide an overview of how the system calculates both relative and absolute quantification of proteins.