Protocol for Fed Batch, Serum Free Cultivation of CHO XM 111 Suspension Cells in the BIOSTAT CultiBag RM 20

In this application note, Sartorius describe a protocol for the propagation of the model protein secreted alkaline phosphatise expressing CHO XM 111 suspension cells (obtained from Prof. Dr. Martin Fussenegger, Swiss Federal Institute of Technology, Zurich) in selective, chemically defined, protein and peptide-free ChoMaster media (HP-1 and HP-5) using the disposable bioreactor BIOSTAT CultiBag RM 20 basic.