Protocol for the Verification of Human Stem Cell Pluripotency

Confirmation of pluripotency is a critical experimental step when studying human embryonic and/or induced pluripotent stem cells. This application note from R&D Systems presents a quick and simple protocol to confirm the ability of your starting cell population to differentiate towards ectoderm, mesoderm and endoderm lineages. Stem cell research studies most often require weeks of cell culture before an experimental hypothesis can be made. The ability to verify pluripotency at an early stage using the Human Pluripotent Stem Cell Functional Identification Kit provides valuable insight that may prevent weeks of wasted effort and reagents.