Prototyping and testing CRISPR technologies with cell-free gene expression

In this application note, Beckman Coulter Life Sciences highlights how Echo Liquid Handlers and cell-free protein expression techniques are used to speed up the prototyping of CRISPR-Cas9 gene circuits. Cell-free transcription/translation (TXTL) is a method for producing proteins from DNA in vitro, gaining popularity across various fields like biology, bioengineering, and synthetic biology. The note highlights its applications in biomanufacturing proteins and prototyping synthetic DNA, enzymes, and regulatory elements.