Purification and Immobilization of a Transaminase for the Preparation of an Enzyme Bioreactor

This application note describes the purification and immobilization of a histidine-tagged transaminase in E. coli using immobilized metal ion affinity chromatography (IMAC) and the ÄKTA™ pure chromatography system with UNICORN™ v6.3 software. The study, which included use of the UNICORN Design of Experiment (DoE) software, showed the proof-of-principle for the application of a Sepharose™ based enzyme bioreactor for industrial-scale synthesis of chemical precursors.