Purification of Adeno-Associated Virus

Adeno-associated viruses (AAV) belong to the family Parvoviridae, subfamily Parvovirus, genus Dependovirus. They range in size from 18-24 nm and are commonly used as gene delivery vectors for human gene therapy. The advantages of AAV as a vector include its lack of pathogenicity, high titer, ease of manipulation, and ability to infect non-dividing cells. This application note demonstrates a one-step method for the purification of a vector using the Sartobind Heparin 75.