Purification of Adeno-Associated Virus using Affinity Chromatography with Sartobind® Membrane Adsorbers

Adeno-associated viruses (AAV) belong to the family Parvoviridae, subfamily Parvovirus, genus Dependovirus. They are used as gene delivery vectors for human gene therapy. The advantages of AAV as a vector include e.g. its lack of pathogenicity, high titer, ease of manipulation, and ability to infect non-dividing cells. This application note describes the purification of adeno-associated virus using affinity chromatography with Sartobind® Membrane Adsorbers.