Purification of Monoclonal Antibodies with BioPro SmartSep

Ion exchange (IEX) is widely used for the analysis and purification of bio-molecules such as proteins, peptides and monoclonal antibodies. For the purification of monoclonal antibodies, high demands are required from the separating material. Factors influencing the binding characteristics of IgG are pH, linear velocity and/or salt concentration (conductivity) at the time the sample is loaded onto the column. This application note investigates the stability of YMC BioPro materials for this use.