Purine nucleotides analyzed with HPLC

In this application note, a method is shown that achieves the separation of Nucleotides XMP from GTP. The analysis of these polar compounds is achieved at a high concentration of an organic Solvent as part of the Mobile Phase which provides increased Sensitivity. Retention times were very Reproducible with a %RSD of approximately 0.4, even when Red Blood Cell extracts were injected in between the standard samples.