Pushing the Limits of Bottom-Up Proteomics with State-Of-The-Art Capillary UHPLC and Orbitrap Mass Spectrometry for Reproducible Quantitation of Proteomes

In this application note, the cooperative use of an EASY-nLC 1200 system with an Orbitrap Fusion Lumos mass spectrometer to separate a HeLa cell lysate is outlined. Since its inception, bottom-up proteomics has aimed to identify and quantify the complete proteome from a cell, tissue or whole organism. Although many advances have been made in the last 15 years, there are still challenges to overcome.