Qualitative and Quantitative Characterization of the Metabolome, Lipidome, and Proteome of Human Hepatocytes Stably Transfected with Cytochrome P450 2E1 Using Data Independent LC/MS

Drug toxicity is a major reason for the failure of candidate pharmaceuticals during their development. Therefore, it is important to realize the potential for toxicity in a timely fashion. In this application note, a label-free multi-omics approach has been applied for the analysis of the THLE transfected human hepatocyte cell line by implementing LC/HDMSE (LC-DIA-IM-MS), providing both qualitative and quantitative information within a single experiment.