Quality control in Illumina sequencing workflows using the TapeStation system

In this application note, Agilent Technologies describes quality control (QC) steps in various Illumina next-generation sequencing (NGS) workflows provided as a service by the German Cancer Research Center (DKFZ) Genomics and Proteomics Core Facility. The QC steps can be performed using the Agilent 4200 TapeStation system with the ScreenTape portfolio. Appropriate reference samples are included as positive controls to monitor the individual steps during library preparation. Representative QC data of these reference samples serve as positive examples for successful completion of critical steps of the most frequently used NGS library protocols.