Quality control in the PacBio HiFi whole genome sequencing library preparation workflow

Accurate and reliable next-generation sequencing (NGS) results rely on starting with high-quality samples. In long-read sequencing workflows, such as the PacBio HiFi workflow, essential quality control (QC) steps include assessing input gDNA, intermediate steps throughout library preparation, and the final library. Electrophoretic analysis enables evaluation of sample size, purity, and integrity at each of these steps. Agilent offers several automated electrophoresis systems, including the Agilent Femto Pulse, Fragment Analyzer, and TapeStation systems, each with unique benefits for DNA QC.

This application note demonstrates the capabilities and limitations of each of the automated electrophoresis systems within the PacBio HiFi whole genome sequencing (WGS) library preparation workflow, aiding in the selection of the appropriate QC system.