Quality control of cell-free DNA samples analyzed with next-generation sequencing

Analysis of cfDNA can introduce challenges due to its low concentration and possible contamination from high-molecular-weight (HMW) DNA, both necessitating reliable quality control. In this application note, Agilent Technologies discusses how to assess the quality of cell-free DNA samples analyzed with next-generation sequencing (NGS). The Agilent TapeStation system is a vital quality control tool in NGS workflows. The TapeStation systems and cell-free DNA ScreenTape assay provide a %cfDNA quality metric for determining the quality of input cfDNA for downstream processes.