Quantification of Protein Samples Using Colorimetric Assays

This application note describes how to use the colorimetric assay applications on the Lunatic systems. Protein quantification is often done by measuring absorbance at 280 nm, results however might be influenced by buffer or source constituents. An alternative to A280 quantification are colorimetric protein assays. Reagent(s) and proteins are mixed, producing a color change which is a measure for the amount of protein present. Unknown sample concentrations are calculated using a measured standard curve.