Quantifying chemotherapeutic cytotoxicity in glial cells using AI-driven label-free cell analysis

In this application note, Sartorius emphasizes the importance of understanding the complex response of tumor cells, particularly glioblastomas, to cytotoxic chemotherapeutic compounds in neuro-oncology research. The Incucyte^® AI Cell Health Analysis Software Module accurately segments and classifies individual cells as live or dead using pre-trained neural networks. This approach enables effective assessment of cytotoxicity in glial cell types and facilitates screening of therapeutic candidates. Furthermore, the challenges in treating glioblastoma multiform (GBM) are discussed, including tumor localization, invasion, heterogeneity, and resistance to conventional therapies.