Quantifying Protein Concentration using UV Absorbance Measured by the Ocean HDX Spectrometer

Proteins are a common sample in clinical, diagnostic and research labs. Studies involving protein extraction, purification or labeling, or working with proteins extracted from cells or labeled for study of the interactions between biomolecules, are common. Determination of protein concentration is a critical part of protein studies. In this application note, we use an Ocean HDX spectrometer to generate a standard curve for bovine serum albumin (BSA). High sensitivity in the UV, ultra-low stray light performance and upgraded optics make the Ocean HDX ideal for UV absorbance measurements.