Quantitation by High Resolution Mass Spectrometry: Using Target Enhancement and Tof-MRM to Achieve Femtogram-level On-column Sensitivity for Quantitation of Drugs in Human Plasma

In this application note the SYNAPT G2-S instrument is used to determine and compare the quantification attributes of detection limit (LOD), quantification limit (LOQ), and linear dynamic range for three data-acquisition modes: full-scan MS, MSE and Tof-MRM. In the presence of human plasma, the Tof-MRM mode of data acquisition can achieve on-column limits of detection that are in the low-femtogram range. Tof-MRM has direct applicability for bioanalysis, targeted metabolism, stability, and PK/PD profiling studies with low detection limits.