Quantitation of 25-Hydroxyvitamin D3 and 25-Hydroxyvitamin D2 in Plasma for Clinical Research Using an Affordable High-Resolution Mass Spectrometer

Analysis of vitamin D2 and vitamin D3 25-hydroxy metabolites (25OHD2 and 25OHD3) in human plasma is one of the highest volume clinical research applications that utilize LC-MS methods. In this application note, a simple, high-efficiency sample preparation method, implemented on a Q Exactive Focus hybrid quadrupole-Orbitrap mass spectrometer, is evaluated.