Quantitation of Sirtuin Activity with a One-Step Luminescent Assay

This poster describes a one-step, homogeneous luminescent assay for the detection of activity for multiple sirtuin isotypes. The Sirtuin family of histone/protein deacetylating enzymes has gained considerable interest both for their recognized importance in processes such as gene silencing and expression and in cellular metabolism. This assay is a convenient and sensitive screening methodology to detect and measure both inhibitors and activators of sirtuin activity.