Quantitative Analysis of 3D Microtissue Growth and Biomarker Intensity

This application note presents a high content screening application to analyze different characteristics of 3D microtissues with the Operetta® High Content Imaging System. The sensitivity of tumor microtissues to treatment with cytotoxic drugs was analyzed, and several cancer associated biomarkers were visualized by staining the microtissues with different in vivo NIR agents. In vivo cells form three-dimensional (3D) microenvironments through cell-cell and cell-matrix interactions to create complex tissues and perform higher level functions. Biochemical signals and nutrient gradients are essential for tissue functioning and therefore also physiological cell behavior.