Quantitative Analysis of Gabapentin from Serum by Reversed Phase HPLC using Discovery C18 Columns

Gabapentin is an analogue of ⁺/_-aminobutyric acid (GABA), a substance approved by the US Food and Drug Administration (FDA) for add-on therapy in treating seizures thought to be associated with interference in GABA-ergic pathways or provoked by excitory amino acids. Gabapentin circulates essentially unbound to serum proteins. This application note describes the use of reversed phase HPLC in the quantitative analysis of Gabapentin extracted from serum.