Quantitative Determination of Serum 25-OH-vitamin D3/D2 using the Tecan AC Extraction Plate™ and the amaZon speed Ion Trap

This application note demonstrates that the Bruker ion trap mass spectrometer amaZon speed is capable of quantifying 25-OH-vitamin D3 and 25-OH-vitamin D2 from serum after sample preparation with the Tecan AC Extraction Plate in the relevant concentration range. 25-OH-vitamin D3 is detected even in the low level calibrator at 4.2 ng/mL with sufficient sensitivity.