Quantitative Live-Cell Analysis for Optimization of Culture Conditions and Evaluation of Cell Health in Human Induced Pluripotent Stem Cell-Derived Neurons

A major limitation in studying human diseases affecting the nervous system is the ability to culture, monitor and analyze neuronal cells that accurately represent human phenotypes of these disorders. The use of human induced pluripotent stem cell (hiPSC)-derived neurons has provided a new approach aimed at modeling neurological diseases. The following application note describes methods and present validation data highlighting optimal culture conditions for evaluation of cell viability and neurite outgrowth in hiPSCderived neurons from Cellular Dynamic International (CDI, iCell Neurons)