RAFT 3D Cell Culture Viability and Proliferation Determination Using the CellTiter-Glo Luminescent Assay

It is a common requirement to assess the viability and proliferation of cells in culture and this is the same in 3D cell culture as it is in planar 2D culture. The CellTiter-Glo luminescent cell viability assay kit from Promega is a popular approach to this requirement in cell biology. In this technical note, it is shown that the standard protocol provided with the kit can be used without modification when some preliminary controls are used with the RAFT 3D cell culture system.