Randomized Adapters for Reducing Bias in Small RNA-Seq Libraries

Read this application note to learn about the Bioo Scientific NEXTflex Small RNA Sequencing Kit v2. While NGS is the most practical method for studies that aim to identify and enumerate small RNAs, bias can skew the resulting data. Bioo Scientific has developed the patent pending NEXTflex Small RNA Sequencing Kit v2 to significantly reduce this bias by incorporating a randomized adapter strategy that involves incorporating pools of adapters with randomized bases at critical positions adjacent to the ligation junctions. This technology substantially reduces the bias typically seen in small RNA sequencing data.