Rapid Assessment of Size Changes Using the Scepter^TMCell Counter can be Predictive of Cell Death

This application note describes a cell counting device used for the analysis of cells undergoing apoptosis. The Scepter^TM handheld, automated cell counter from Millipore uses impedance-based particle detection to reliably and precisely count every cell in a sample. Precise volumes of single-cell suspensions are drawn into the sensor at the end of the Scepter device. Using NIH 3T3 and CHO cells, Millipore demonstrate that the relationship between apoptosis and cell volume depends on cell type and a specific apoptosis-inducing agent.