Rapid high-throughput profiling and quantitation of sialic acids in biotherapeutics

This poster presents a new high-throughput workflow based on a 96-well plate format for the release, labeling, and analysis of sialic acids from therapeutic glycoproteins using rituximab, etanercept, and NISTmAb as examples. Sialic acid residues are released then labeled with 1,2-diamino-4,5methylenedioxybenzene (DMB) in a two-step procedure.