Rapid His-Tag Purification of Recombinant Proteins Using Dionex ProPac IMAC Columns

This application note demonstrates that the Dionex ProPac IMAC column can be used to successfully and rapidly purify His-Tagged recombinant proteins from cell-free extracts harvested from up to 1 L of E. coli fermentation cultures. Purification time may be reduced further through the application of a more concentrated sample with a smaller sample volume.