Rapid Improvements for LC/MS/MS Analysis without Additional Method Development Using Phree™ Phospholipid Removal Plates

In this application note, protein precipitation is compared to a phospholipid removal product, Phree Phospholipid Removal Plates, to assess the clean up capabilities of each technique. LC/MS/MS analysis of the phospholipids present each sample indicates that Phree Phospholipid Removal Plates result in significantly cleaner samples, reduced ion suppression, and extended HPLC/UHPLC column lifetime. Phree Phospholipid Removal is a simple procedure similar to protein precipitation, requires no method development and can be automated to provide higher throughput.