Rapid, Label-free, Direct Cell Counting in Measurement of Cell Proliferation for Compound Screening

Cell proliferation is one of the most widely used assay types across a broad range of biological disciplines. Due to their sensitive nature, cell proliferation assays are commonly used to measure cytotoxicity and/or efficacy of drug candidates in oncology, immunology, and stem cells, among other fields. This application note demonstrates the use of the Celigo cytometer from Brooks Life Science Systems in a label-free, cell-based screening assay to directly monitor cell proliferation. The Celigo cytometer produced similar results to MTT assays, but with several advantages, such as image archiving and kinetic growth data.