Rapid LC-MS/MS method for monitoring bio-relevant levels of per- and polyfluoroalkyl substances (PFAS) in serum

In this application note, a quantitative workflow for the analysis of 22 per- and polyfluoroalkyl substances (PFAS) in serum was developed using a Gemini^™ 3µm C18 column coupled with a SCIEX QTRAP^® 6500+system. This targeted screening workflow provides a fast analytical method capable of accurately quantifying sub-nanogram per mL levels of PFAS in the human body.

Per- and polyfluoroalkyl substances are pervasive compounds used in a variety of industrial applications and found in a wide range of consumer products such as cookware, stain repellent, flame-retardant, and coatings. PFAS are considered environmental factors due to their persistent and bio accumulating nature. Bioaccumulation of PFAS in the human body resulting from environmental exposure is a growing public health concern. Recent studies have linked PFAS exposure to adverse health outcomes including:

• childhood health complications
• reduced kidney function
• thyroid disease
• hormone suppression
• decreased fertility
• increased cholesterol levels
• diabetes