Rapid Measurement of IgG Using Fluorescence Polarization

The accurate, rapid and high-throughput measurement of IgG is essential in the development, and the subsequent manufacture of most therapeutic antibodies, as monoclonal antibodies are becoming increasingly dominant in biopharmaceuticals. In this application note, we describe the use of the Synergy™ Neo2 Hybrid Multi-Mode Reader to rapidly determine IgG concentrations using the fluorescence polarization based Valita™TITER assay kit provided by Valitacell.