Rapid qPCR Analysis from Highly Inhibited Tissue and Blood Sample Extractions

Enzymatic inhibition, caused by both sample and extraction carryover inhibitors, is a significant obstacle when optimizing crude sample workflows. KAPA PROBE FORCE qPCR Kits include a highly processive third-generation enzyme—engineered via directed evolution—which enables direct crude sample analysis without sample purification. This application note demonstrates that, in combination with KAPA Express Extract, KAPA PROBE FORCE offers a highly efficient and streamlined sample-to-Cq workflow solution for both low- and high-throughput genotyping and gene expression applications (<1.5 hr).