Rapid, Reliable Quantitation of Fc-Fusion Protein in Cell Culture Supernatants

Biogen IDEC's cell line development group needed a robust assay for the measurement of Fc-fusion protein in crude cell culture supernatants. The group had historically used HPLC for protein quantitation during screening and selection of promising mammalian clones at every scale-up step, from 96-well microplates to 3L-bioreactors. They wanted to replace the HPLC method due to its low throughput, cumbersome sample processing, and long run times with a higher-throughput alternative that provided accurate, reliable results. This application note summarizes the performance of the new protein assay that was developed.