Rapid Screening of Sialic Acids in Glycoproteins by HPAE-PAD

In this application note, sialic acids are determined in five representative glycoproteins by acid hydrolysis followed by HPAE-PAD. Sialic acid determination by HPAE-PAD using the Thermo Scientific Dionex CarboPac PA20 Fast Sialic Acid column is specific and direct, eliminating the need for sample derivatization after hydrolysis. The use of a disposable gold on polytetrafluoroethylene (PTFE) working electrode simplifies system maintenance while providing consistent response with a lifetime of four weeks.