Rapid Solution Exchange for Recording of Fast-Desensitizing Ligand-Gated Ion Channels on the IonWorks Barracuda System

In this study the real-time change of Kv1.3 current in voltage-clamped cells is measured after the regular external buffer was replaced by buffer containing high potassium (high K+). The experimental design removes any acceleration of current kinetics caused by ionic current passing through partially activated channels as would be the case with partially activated ligand gated ion channels. Recordings are also presented of a7-nAChR and a1-nAChR currents.