Real-time cell motility tracking increases the throughput of scratch wound assays

In this application note, BMG LABTECH discusses the use of microplate readers for measuring the motility of cells, specifically in the context of wound healing and invasion assays. The note describes important factors to consider when performing these assays, such as the choice of a proper cell line, the selection of the right assay format, and the attainment of proper cell confluency prior to the assay. The note also discusses the use of key detection technologies such as fluorescence, luminescence, and absorbance, comparing the benefits and drawbacks of each technology.