Real-Time Cytotoxicity Analysis Using CellTox™ Green Dye

This application note demonstrates the use of a non-activity-based cytotoxicity probe, CellTox™ Green Dye, which can be added at the beginning of an experiment and employed in real time for the kinetic determination of cytotoxicity. The HP D300 Digital Dispenser, available from Tecan, was used for noncontact dispensing of test compound and the Tecan Infinite® M200 PRO with Gas Control Module (GCM™) for kinetic measurement of cytotoxicity in HepG2 cells over 72 hours.