Real-Time Visualization and Quantification of Neutrophil Extracellular Traps

Expulsion of extracellular traps is a defense mechanism utilised by neutrophils upon contact with microbes. Extracellular Traps are formed of DNA and antimicrobial proteins including myeloperoxidase (MPO) and neutrophil elatase (NE). This poster describe a simple kinetic live-cell imaging approach using a combination of phase and fluorescence imaging to visualise stages of neutrophil extracellular trap (NET) formation.