REAlease Fluorochrome Technology for Multiparameter Cell Sorting

Multiparameter immunofluorescent labeling is the method of choice for sorting of cell populations out of heterogeneous mixtures. However, downstream applications of isolated cells are usually limited since fluorescence channels and epitopes are blocked by the antibody fluorochrome conjugates utilized for the flow sorting experiment. Accordingly, antibody fluorochrome conjugates that would allow for a release of cell epitopes and a reuse of fluorescence detection channels after cell sorting might be of high interest for certain downstream applications.