Reduction of Matrix Effects Using LC-MS/MS with Online Extraction for Quantitation of Antibiotics in Milk

In this application note, a study was carried out using the Bruker EVOQ Elite liquid chromatography mass spectrometer (LC-MS/MS) with an online extraction (OLE) option to quantify 8 antibiotics in milk. The milk matrix clean-up was performed online using an innovative UHPLC integrated with a binary pump and one loading pump. The EVOQ Elite with OLE showed a significant reduction of matrix effects and excellent linearity, with online extraction providing an effective, timesaving approach for clean up.