Repeatability and Quantitation in a Method Using the Alliance HPLC System with the 2998 Photodiode Array Detector and the ACQUITY QDa Detector

In this application note, Waters demonstrates orthogonal detection techniques – namely, PDA and mass detection – in combination with HPLC, to identify and quantitate two compounds. This chromatographic configuration produced the required area and retention time reproducibility, in addition to reliable quantitation, peak identity, and homogeneity.