Resolution Of Normal Phase Flash Chromatography Improved by Dry Samples

This application note demonstrates that preparing dry samples using RediSep® solid load sample cartridges improves peak resolution over liquid injection techniques. When the compound and reaction by-products are over-diluted or dissolved in too polar a dissolving solvent, the compound peak broadens which leads to loss of resolution. The effects of band broadening and overlapping peaks were gauged using dual UV detection/monitoring.